Antimicrobial and Immunomodulatory Action of Probiotic Composition of Bacilli on Bacterial Vaginitis in Mice

The purpose of this study was to investigate the antimicrobial and immunomodulatory action a probiotic composition Bacillus subtilis B. megatherium strains (UnicaUro, Sirion (Ukraine)) for experimental bacterial vaginitis. Methods. Experimental studies were conducted on female BALB/c mice; we used Staphylococcus aureus strain B-918 (ATCC 6538) induce vaginally introduced into mice before treatment with bacteria. In vagina mice, aerobic optionally anaerobic bacteria, including representatives genera Staphylococcus, Streptococcus, Lactobacillus, Bifidobacterium, Pseudomonas, coliform microscopic fungi identified in different periods observation using generally accepted microbiological methods. Serum antibody titer S. determined by agglutination reaction. phagocytic activity oxygen-dependent bactericidal peritoneal exudate macrophages (PEM) evaluated immunological Results. formation vaginitis line infected evidenced appearance external clinical manifestations infectious inflammatory process against background increased number microorganisms, genus fungi, decreased lactobacilli periods. introduction led dynamic change vaginal microbiota: microorganisms decreased, primarily due normalization accompanied decrease staphylococcus blood serum. effective therapeutic confirmed gradual disappearance signs infectious-inflammatory functional PEM. Conclusions. Sirion, Ukraine) is promising formulation that may be vaginitis; however, further are required confirm its therapeutic, antimicrobial, efficacy.